The Center for Environmental Diagnostics and Bioremediation (CEDB) is located at the main campus of the University of West Florida (UWF). CEDB's administrative and faculty offices and research laboratories are located in the biology/chemistry building (Bldg. 58). CEDB has additional office space in an adjacent modular facility (Bldg. 6) and a separate analytical services laboratory (Wetlands Research Laboratory; Bldg. 83).
Currently CEDB has five research laboratories (total space of ~3,500 sq. ft.) equipped to carry out studies in diverse areas.
The available instruments include: Polaroid Camera System; Fotodyne gel image analysis system; 2 Corbett Research Rotor-Gene R6.3000 Q-PCR machines; 4 Perkin Elmer GeneAmp 2400 and one Perkin Elmer GeneAmp 9700 PCR Systems; PCR hoods, electrophoresis units; western blotting system; electroporation apparatus; ABI Avant 3100 automated DNA sequencer with Genescan and sequencing software; refrigerated Sorvall high speed and Sorvall RC M15GX Micro-Ultra-centrifuges; bench top centrifuges; UV-Vis spectrophotometers; Genequant Pro Classic RNA/DNA CALC/ spectrophotometer; Spectrofluorometer; Anaerobic chamber, and anaerobic gassing manifold; oxygen, pH, redox electrodes; Hydrolab DataSondes; hand-held water quality multimeters; Fisher Tensiomat surface tensiometer; refrigerators, -20 °C and -70 °C freezers; Turner designs TD-700 fluorometer; Biospherical Instruments GUV511C and PUV500 ultraviolet radiometers, chromatography equipment and fraction collectors; water purification systems; analytical balances; pH meters; lyophilizer; shakers and incubators; laminar flow hood; rotary evaporators; nitrogen evaporators; rotary hybridization ovens; compound and binocular microscopes; epifluorescence microscope, and an inverted microscope; vacuum pumps; and desiccators.
Additional general facilities available in the biology-chemistry building include:
radiochemistry laboratory; analytical chemistry laboratory equipped with chemical fume hoods;
H-1 flammable storage facilities; electron microscopy laboratory; dark rooms; walk-in environmental chambers and cold rooms; dish-washing facilities; autoclaving facilities; biological and radiochemical waste disposal services; and a stock room for general glassware and chemicals.
The Wetlands Research Laboratory (WRL, Bldg. 83), a 3500 sq. ft. facility, is an analytical arm of CEDB. The WRL is State of Florida Certified for environmental water analysis (Lab ID:E71176), conforming to the standards set out by the National Environmental Laboratory Accreditation Conference as adopted into Chapter 64E-1 of the Florida Administrative Code. The laboratory complies with full chain of custody sample storage and handling practices.
The WRL is equipped with pH, salinity, conductivity, and dissolved oxygen meters, ovens, incubators, a muffle furnace, analytical and top-loading balances, an autoclave, water baths, block digesters, sonicator,
research-grade (Leica) compound and dissecting microscopes, a Hewlett-Packard gas chromatographs (5890 Series II) with flame-ionization and electron capture-detectors with autosampling and Chemstation control, a GC-Mass spectrometer (Finnigan/Thermoquest Trace 2000) with autosampler and Tekmar purge and trap, a Varian SPECTRAA 220FS flame atomic absorption spectrometer (AAS), a Varian SPECTRAA 220Z Zeeman furnace AAS, a Dionex DX500 ion chromatograph, a Bran and Luebbe AutoAnalyzer 3 nutrient analyzer with autosampler, a ThermoFinnigan Flash EA Elemental Analyzer, and a CETAC Technologies M-6000A Cold Vapor mercury analyzer with auto sampler.
Field equipment includes: Dodge Ram Van (1996), Dodge Dakota Truck (2002), 28- foot pontoon boat, two Jon boats and a 200 hp 21 ft Cape Horn Center console craft capable of sampling up to 20 miles offshore. It also has gasoline powered field generators, an enclosed 24-foot mobile lab, and sediment and water sampling equipment.
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