Wade H. Jeffrey

Selected Publications

Joux, F., W.H. Jeffrey, M. Abboudi, J. Neveux, M. Pujo-Pay, L. Oriol, and J-J. Naudin. Ultraviolet radiation in the Rhône River lenses of low salinity and in marine waters of the northwestern Mediterranean Sea: attenuation and effects on bacterial production and net community production. Submitted (Photochemistry Photobiology).

Neale, P. J., W. H. Jeffrey, C. Sobrino, J. D. Pakulski, J. D.Phillips-Kress, A. J. Baldwin, L. A. Franklin, and H. Kim. 2008. Inhibition of Phytoplankton and Bacterial Productivity by Solar Radiation in the Ross Sea Polynya. in M. Lang, S. Miller, and I. Krupnik, editors. Smithsonian at the Poles. Contributions to IPY Science. Smithsonian Press, Washington, D.C. (In press)

Pakulski. J.D., J.A. Meador, J.P. Kase, and W.H. Jeffrey. 2008. Effect of stratospheric ozone depletion and enhanced ultraviolet radiation on marine bacteria at Palmer Station, Antarctica in the early austral spring. Photochemistry and Photobiology 84: 215-221.

Abboudi, M., W.H. Jeffrey, J.-F. Ghiglione, M. Pujo-Pay, L. Oriol, R. Sempéré, B. Charrière, and F. Joux. 2007. Effects of Photochemical Transformations of Dissolved Organic Matter on Bacterial Metabolism and Diversity in Three Contrasting Coastal Sites in the Northwestern Mediterranean Sea. Microbial Ecology 55: 344-357.

Pakulski, J.D., A. Baldwin, A. Dean, S. Durkin, D. Karentz, C.A. Kelley, K. Scott, H. J. Spero, S. Wilhelm and W. H. Jeffrey. 2007. Responses of heterotrophic bacteria to latitudinal variation in solar irradiance. Aquatic Microbial Ecology 47:153-162.

Mioni, C.E., A.J. Baldwin, M.R. Twiss, L. Poorvin, J.D. Pakulski, W.H. Jeffrey, and S.W. Wilhelm. 2007. Variability in the in situ bioavailability of Fe to bacterioplankton communities in the eastern subtropical Pacific Ocean. Aquatic Microbial Ecologyy 46: 239-251.

Hoch, M.P., W.H. Jeffrey, R.A. Snyder, K. Dillon, and R.B. Coffin. 2006. Expression of glutamine synthetase and glutamate dehydrogenase by marine bacterioplankton: assay optimizations and efficacy for assessing nitrogen to carbon metabolic balance. Limnology and Oceanography: Methods 4: 294-307.

Baldwin. A.J., J.A. Moss, J.D. Pakulski, P. Catala. F. Joux, and W.H. Jeffrey. 2005. Microbial diversity in a Pacific Ocean transect from the Arctic to Antarctic circles. Aquatic Microbial Ecology 41: 91-102.

Mitchell, D.L., and W.H. Jeffrey. 2005. Can you really put something where the sun don’t shine? Journal of Irreproducible Results 49: 6- 10.

Arnosti, C., S. Durkin, and W.H. Jeffrey. 2005. Patterns of extracellular enzyme activities among pelagic microbial communities: Implications for cycling of dissolved organic carbon. Aquatic Microbial Ecology 38: 135-145.

MacFadyen, E.J., C.E. Williamson, G. Grad, M. Lowery, W.H. Jeffrey, and D.L. Mitchell. 2004. Molecular Response to Climate Change: Temperature Dependence of UV-induced DNA Damage and Repair in the Freshwater Crustacean Daphnia pulicaria. Global Change Biology 10: 408-416.

Simmon, K., D. Steadman, S. Durkin, A. Baldwin, W.H. Jeffrey, P. Sheridan, R. Horton and M.S. Shields. 2004. An autoclave method for rapid preparations for bacterial PCR template DNA. Journal of Microbiological Methods. 56: 143-149.

Wilhelm, S., W.H. Jeffrey, A. Dean, J. Meador, and D.L. Mitchell. 2003. Ultraviolet radiation induced DNA damage in marine viruses along a latitudinal gradient. Aquatic Microbial Ecology 31: 1-8.

Buma, A.G.J., P. Boelen and W.H. Jeffrey. 2003. UVR-induced DNA damage in aquatic organisms. In: W. Helbling and H. Zagarese (eds.), Comprehensive series in Photosciences “UV effects in aquatic organisms and ecosystems.” The Royal Society of Chemistry, Cambridge, UK. pp. 291-327

Wilhelm, S.W., W.H. Jeffrey, and C.A. Suttle, and D.L. Mitchell. 2002. Estimation of Biologically Damaging UV levels in Marine Surface Waters with DNA and Viral Dosimeters. Photochemistry and Photobiology 76(3): 268 - 273.

Kelley, C.A., and W.H. Jeffrey. 2002. Dissolved methane concentrations profiles and air-sea fluxes from 41° S to 27° N. Global Biogeochemical Cycles. 10.1029/2001GB001809.

Mitchell, D.L, J. Meador, L. Paniker, D. Gasparutto, W.H. Jeffrey, and J. Cadet. 2002. Development and Application of a Novel Immunoassay for Measuring Oxidative DNA Damage in the Environment. Photochemistry and Photobiology 75: 257-265.

Meador, J., W. H. Jeffrey, J. P. Kase, J. D. Pakulski, S. Chiarello1, and D. L. Mitchell. 2002. Seasonal fluctuation of DNA photodamage in Plankton Assemblages at Palmer Station, Antarctica. Photochemistry and Photobiology 75: 266-271.

Jeffrey, W.H., and D.L. Mitchell. 2001. Measurement of UVB-induced DNA damage in marine planktonic communities. In J.H. Paul (ed). Methods in Microbiology: Marine Microbiology. Academic Press, New York. 30: 469-485.

Booth, M.G., W.H. Jeffrey, and R.V. Miller. 2001. RecA expression in response to solar ultraviolet radiation in the marine bacterium Vibrio natriegens. Microb. Ecol. 42:531-53..

Booth, M.G, L. Hutchinson, M. Brumsted, P. Aas, R. B. Coffin, R. C. Downer, Jr, C. A. Kelley, M. M. Lyons, J. D. Pakulski, S. L. Holder Sandvik , W. H. Jeffrey, and R. V. Miller. 2001. Quantification of RecA as an Indicator of Repair Potential in Marine Bacterioplankton Communities of Antarctica. Aquatic Microbial Ecology 24: 51-59.

Jeffrey, W.H. And D.L. Mitchell. Measurment of UVB induced DNA damage in marine planktonic communities. 2001. In J. Paul (ed) Methods in marine microbiology. Vol. 30. Academic Press, NY. pp. 469-485.

Kepner, R.L., R.A. Wharton, R. Collier, C. Cockell, and W.H. Jeffrey. 2000. UV radiation and potential effects beneath the perennial ice cover of an Antarctic lake. Hydrobiologia. 427:155-165.

Huot, Y., J.J. Cullen, W.H. Jeffrey, and R.F. Davis. 2000. Damage to DNA in bacterioplankton: a model of damage by ultraviolet radiation and its repair as influenced by vertical mixing. Photochemistry and Photobiology 72:62-74.

Jeffrey, W.H. J.P. Kase, and S.W. Wilhelm. 2000. Ultraviolet Radiation Effects on Bacteria and Viruses in Marine Ecosystems. In S. de Mora, S. Demers, and M. Vernet (ed). The Effects of UV Radiation on Marine Ecosystems. Cambridge University Press. pp. 206-236.

Kelley, C.A, J.D. Pakulski, S.H. Sandvik, R.B. Coffin, R.C. Downer, Jr., P. Aas, M.M. Lyons, and W.H. Jeffrey. 1999. Phytoplanktonic and bacterial carbon pools and productivities in the Gerlache Strait, Antarctica during early austral spring. Microb. Ecol. 38: 296-305.

Joux, F., W.H. Jeffrey, P. Lebaron, and D. Mitchell. 1999. Marine Bacteria Display Diverse Responses To Ultraviolet-B Radiation. Appl. Environ. Microbiol. 65: 3820-3827.

Miller, R.V., W. Jeffrey, D. Mitchell, and M. Elasri. 1999. Bacterial responses to Solar Ultraviolet light. ASM News. 65: 535-541.

Massana, R. L. T. Taylor, A.E. Murray, K. Y. Wu, W.H. Jeffrey, and E. F. DeLong. 1998. Distribution of marine planktonic Archaea in the Gerlache Straight, Antarctic Peninsula, during early spring. Limnology and Oceanography 43: 618-624.

Wilhelm, S.W., M.G. Weinbauer, C.A. Suttle, and W.H. Jeffrey. 1998. The role of sunlight in the removal and repair of viruses in the sea. Limnology and Oceanography 43: 586-592.

Lyons, MM., P. Aas, J.D. Pakulski, L. Van Waasbergen, D.L. Mitchell, R.V. Miller, and W.H. Jeffrey. 1998. Ultraviolet radiation induced DNA damage in coral reef microbial communities. Mar. Biol. 130: 537-543.

Jeffrey, W.H. 1998. Induction of DNA Photodamage in Marine Microbial Communities By Ultraviolet Radiation. In H. Honigsmann, R.M. Knobler, F. Trautinger, and G. Jori (eds) Proceedings of the 12th International Congress on Photobiology. Association Internationale de Photobiologie. Organizzazione Editoriale Medico Farmaceutica, Milano, Italy. pp. 26-30.

Pakulski, J.D., P. Aas P, W.H. Jeffrey, M. Lyons, L. Von Waasenbergen, D. Mitchell, and R. Coffin. 1998. Influence of light on bacterioplankton production and respiration in a subtropical reef. Aquat. Microb. Ecol. 14:137-148.

Jeffrey, W.H., R.V. Miller, and D.L. Mitchell. 1997. Detection of ultraviolet radiation induced DNA damage in microbial communities of the Gerlache Strait. The Antarctic Journal of the United States. 32: 85-87.

Booth, C.R., J.H. Morrow, T.P. Coohill, J.J. Cullen, J.E. Frederick, D-P. Häder, O. Holm-Hansen, W. H. Jeffrey, D.L. Mitchell, P.J. Neale, I. Sobolev, J. Van Der Leun, and R.C. Worrest. 1997. Impacts of Solar UVR on Aquatic Microorganisms. Photochem. Photobiol. 65: 252-269.

Aas, P., M. M. Lyons, R. J. Pledger , D.L. Mitchell, and W.H. Jeffrey. 1996. Inhibition of Bacterial Activities by Solar Radiation in Nearshore Waters and the Gulf of Mexico. Aquat. Microb. Ecol. 11: 229-238.

Jeffrey, W.H., P. Aas, M. M. Lyons, R. Pledger, D.L. Mitchell, and R. B. Coffin. 1996. Ambient Solar Radiation Induced Photodamage in Marine Bacterioplankton. Photochem. Photobiol. 64 (3): 419-427.

Pakulski, J.D., R.B. Coffin, C.A. Kelley, R. Downer, M. Maille Lyons, P. Aas, and W.H. Jeffrey. 1996. Iron stimulation of Antarctic bacteria. Nature 383: 133-134

Jeffrey, W.H., R. Von Haven, M.P. Hoch, and R.B. Coffin. 1996. Bacterioplankton RNA, DNA, protein content and relationships to rates of thymidine and leucine incorporation. Aquatic Microbial Ecology 10: 87-95.

Jeffrey, W.H., S. Nazaret, and T. Barkay. 1996. Detection of the merA gene and its expression in the environment. Microb. Ecol. 32: 293-303.

Jeffrey, W.H., R.J. Pledger, P. Aas, S. Hager, R.B. Coffin, R. Von Haven, and D.L. Mitchell. 1996. Diel and depth profiles of DNA photodamage in bacterioplankton exposed to ambient solar ultraviolet radiation. Mar. Ecol. Progr. Ser. 137: 293-304.

Jeffrey, W.H., D.L. Mitchell, and R.B. Coffin. 1995. Molecular studies of ultraviolet radiation effects on marine bacteria, pp. 90-104. In: M. Levin, C. Grim, and J.S. Angle (eds.), Biotechnology Risk Assessment: Risk Assessment Methodologies, Proceeding of the Biotechnology Risk Assessment Symposium. Univ. of MD Biotechnology Institute, College Park.

Barkay, T., S. Nazaret, and W.H. Jeffrey. 1995. Degradative genes in the environment. In: L.Y. Young and C. Cerniglia (ed) Microbiological transformation and degradation of toxic organic chemicals. Wiley-Liss, Inc., New York. pp. 545-577.

Santavy, D.L., W.H. Jeffrey, R.A. Snyder, J. Campbell, P. Malouin, and L. Cole. 1994. Microbial community dynamics in the mucus of healthy and stressed coral hosts. Bull. Mar. Sci. 54: 1077-1078.

Nazaret, S., W.H. Jeffrey, E. Saouter, R. VonHaven, and T. Barkay. 1994. mer gene expression in aquatic environments measured by mRNA production and Hg(II) volatilization. Appl. Environ. Microbiol. 60: 4059-4065.

Jeffrey, W.H., S. Nazaret, and R. Von Haven. 1994. Improved method for the recovery of mRNA from aquatic samples: application to detecting mer gene expression. Appl. Environ. Microbiol. 60: 1814-1821.

Jeffrey, W.H., S. M Cuskey, R. B. Coffin, and M. Reagin. 1992. The use of lethal bacterial genes to limit the survival of intentionally released genetically engineered microorganisms, pp. 225-227. In Proceedings of the 4th investigators' meeting for EPA's biotechnology-biological control agent risk assessment research program. United States Environmental Protection Agency, Washington, DC. EPA/600/R-92/147.

Jeffrey, W.H., S.M. Cuskey, P.J. Chapman, S. Resnick, and R.H. Olsen. 1992. Pseudomonas putida mutants unable to catabolize benzoate: Cloning and characterization of Pseudomonas genes involved in benzoate catabolism and isolation of a chromosomal DNA fragment able to substitute for xylS in the activation of the TOL lower pathway promoter. J. Bacteriol. 174: 4986-4996.

Jeffrey, W.H., and J.H. Paul. 1990. Thymidine uptake, thymidine incorporation, and thymidine kinase activity in marine bacterium isolates. Appl. Environ. Microbiol. 56: 1367-1372.

Paul, J.H., L.H. Cazares, A.W. David, M.F. DeFlaun, and W.H. Jeffrey. 1991. The distribution of dissolved DNA in oligotrophic and eutrophic rivers in southwest Florida. Hydrobiologia 218: 53-63.

Paul, J.H., W.H. Jeffrey, and J.P. Cannon. 1990. Production of dissolved DNA, RNA, and protein by microbial populations in a Florida reservoir. Appl. Environ. Microbiol. 56: 2957-2962.

Jeffrey, W.H., J.H. Paul, L.H. Cazares, M.F. DeFlaun, and A.W. David. 1990. Correlation of non-specific macromolecular labeling with environmental parameters during [3H]thymidine incorporation in the waters of southwest Florida. Microb. Ecol. 20: 21-35.

Jeffrey, W.H., J.H. Paul, and G.J. Stewart. 1990. Natural transformation of a marine Vibrio sp. by plasmid DNA. Microb. Ecol. 19: 259-268.

Paul, J.H., W.H. Jeffrey, A.W. David, M.F. DeFlaun, and L.H. Cazares. 1989. The turnover of extracellular DNA in eutrophic and oligotrophic freshwater environments of southwest Florida. Appl. Environ. Microbiol. 55: 1823-1828.

Jeffrey, W.H., and J.H. Paul. 1988. Underestimation of DNA synthesis by [3H]thymidine incorporation in marine bacteria. Appl. Environ. Microbiol. 54: 3165-3168.

Paul, J.H., M.F. DeFlaun, and W.H. Jeffrey. 1988. Mechanisms of DNA utilization by estuarine microbial populations. Appl. Environ. Microbiol. 54: 1682-1688.

Paul, J.H., M.F. DeFlaun, W.H. Jeffrey, and A.W. David. 1988. Seasonal and diel variability in dissolved DNA and in microbial biomass and activity in a subtropical estuary. Appl. Environ. Microbiol. 54: 718- 727.

Jeffrey, W.H., and J.H. Paul. 1988. The effect of 5-fluoro 2'-deoxyuridine on [3H]thymidine incorporation by bacterioplankton in the waters of southwest Florida. Appl. Environ. Microbiol. 54: 331-336.

DeFlaun, M.F., J.H. Paul, and W.H. Jeffrey. 1987. Distribution and molecular weight of dissolved DNA in subtropical estuarine and oceanic environments. Mar. Ecol. Progr. Ser. 34: 65-73.

Paul, J.H., W.H. Jeffrey, and M.F. DeFlaun. 1987. The dynamics of extracellular DNA in the marine environment. Appl. Environ. Microbiol. 53: 170-179.

Jeffrey, W.H., and J.H. Paul. 1986. The activity of attached and free-living Vibrio sp. as measured by thymidine incorporation, p-iodonitrotetrazolium reduction, and ATP/DNA ratios. Appl. Environ. Microbiol. 51: 150-156.

Paul, J.H., and W.H. Jeffrey. 1985. The effect of surfactants on the attachment of estuarine and marine bacteria to surfaces. Can. J. Microbiol. 31: 224-228.

Paul, J.H., M.F. DeFlaun, and W.H. Jeffrey. 1986. Elevated levels of microbial activity in the coral surface microlayer. Mar. Ecol. Progr. Ser. 33: 29-40.

Jeffrey, W.H., and J.H. Paul. 1986. Activity measurements of planktonic microbial and microfouling communities in a eutrophic estuary. Appl. Environ. Microbiol. 51: 157-162.

Jeffrey, W.H., and J.H. Schiltz. 1985. Algaecides: Solutions or problems? Pool and Spa News 24: 154-156.

Paul, J.H., W.H. Jeffrey, and M.F. DeFlaun. 1985. Particulate DNA in subtropical oceanic and estuarine planktonic environments. Mar. Biol. 90: 95-101.

Paul, J.H., and W.H. Jeffrey. 1985. Evidence for separate adhesion mechanisms for hydrophilic and hydrophobic surfaces in Vibrio proteolytica. Appl. Environ. Microbiol. 50: 431-437.

Paul, J.H., and W.H. Jeffrey. 1984. Measurements of diameters of estuarine bacteria and particulates in natural water samples by use of a submicron particle analyzer. Curr. Microbiol. 10: 7-12.