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Authors: JinSeup Lim
, Angelina Sansone
, Yi-Chun Huang
, Venkat Sharma
Abstract:
Peripheral Blood Mononuclear Cells (PBMC) comprise the basis of a number of studies done
in our laboratory. PBMC are typically isolated from whole blood by centrifugation and
phase separators. These methods limit the yield of PBMC. New research suggests the use of
filters that are employed in blood bank prior to transfusion to increase number of PBMC
isolated. These filters have shown to isolate over 99% of the PBMC. The PBMC isolated
will provide us with a pure sample for further isolation of a specific type of B-cells,
called CD-19 Cells.
These CD-19 cells are the leading source of cells in our studies to understand
AIDS-associated B-Cell malignances. Furthermore, these purified CD-19 B-Cells help to
investigate the role of retro-viral proteins in modulating several B-Cell cytokine genes.
Modulations in B-Cell cytokine genes partially explain the molecular mechanism of
cytokine involvement in AIDS related B-Cell lymphomas. Understanding the mechanism(s) of
cytokine regulation will pave way for novel drug development against AIDS-associated
B-cell lymphomas.
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